Expression of catalytically active rat S‐adenosylmethionine decarboxylase in Escherichia coli

FEBS Letters

Pajunen, Antti¹  Salmikangas, Paula¹  Keranen, Marja-Riitta¹ 

[1] Biocenter and Department of Biochemistry, University of Oulu, SF-90570 Oulu, Finland

关键词: S-Adenosylmethionine decarboxylase;    DNA;    recombinant;    Expression;    (E. coli;    Rat);   

DOI  :  10.1016/0014-5793(89)81631-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cDNA coding for rat S-adenosylmethionine decarboxylase (AdoMetDC, EC 4.1.1.50) has been cloned into a plasmid expression vector, pKK-223-3, and expressed in E. coli. The authenticity of the expressed protein has been demonstrated by reactivity with antibodies specific for rat Ado-MetDC, by size analysis on SDS gels visualized with immunotransblots, and, finally, by catalytic activity. The expression of the enzyme results in a decrease in the activity of the bacterial enzyme suggesting the replacement of the bacterial enzyme by the rat AdoMetDC. Similarly, the addition of exogenous spermidine to the growth medium reduces bacterial enzyme activity affecting only marginally the expression of the recombinant protein.

【 授权许可】

Unknown