FEBS Letters | |
Construction of a heat‐inducible chimaeric gene to increase the cytokinin content in transgenic plant tissue | |
Van Onckelen, H.1  Beinsberger, S.1  Spena, A.2  Schmülling, T.2  Schell, J.2  De Greef, J.1  | |
[1] Dept of Biology, UIA, B-2610 Wilrijk, Belgium;MPI für Züchtungsforschung, 5000 Köln 30, FRG | |
关键词: Heat shock; Cytokinin; DNA; recombinant; Transgenic plant; CK; cytokinin; [9R]ip; N 6(Δ2-isopentenyl)adenosine; ip; N 6 (Δ2-isopentenyl)adenine; [9R]Z; zeatinriboside; Ab; antibody; PVP; polyvinylpyrrolidone; RIA; radioimmunoassay; | |
DOI : 10.1016/0014-5793(89)80667-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The ipt gene of Agrobacterium tumefaciens T-DNA encodes an isopentenyltransferase which causes cytokinin overproduction and developmental alterations in transformed plants. A chimaeric gene constructed by positioning the ipt coding region under the control of the hsp70 gene from Drosophila melanogaster allows heat-regulated expression in transgenic plant tissue. Heat-shock treatment of tobacco calli transgenic for the chimaeric hsipt gene increases the endogenous cytokinin concentration and enables these calli to grow on cytokinin-free medium. Transgenic plants regenerated from calli transformed with the hsipt gene and grown at normal temperature are phenotypically normal.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020292108ZK.pdf | 475KB | download |