Construction of a heat‐inducible chimaeric gene to increase the cytokinin content in transgenic plant tissue

FEBS Letters

Van Onckelen, H.¹  Beinsberger, S.¹  Spena, A.²  Schmülling, T.²  Schell, J.²  De Greef, J.¹ 

[1] Dept of Biology, UIA, B-2610 Wilrijk, Belgium;MPI für Züchtungsforschung, 5000 Köln 30, FRG

关键词: Heat shock;    Cytokinin;    DNA;    recombinant;    Transgenic plant;    CK;    cytokinin;    [9R]ip;    N 6(Δ2-isopentenyl)adenosine;    ip;    N 6 (Δ2-isopentenyl)adenine;    [9R]Z;    zeatinriboside;    Ab;    antibody;    PVP;    polyvinylpyrrolidone;    RIA;    radioimmunoassay;   

DOI  :  10.1016/0014-5793(89)80667-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ipt gene of Agrobacterium tumefaciens T-DNA encodes an isopentenyltransferase which causes cytokinin overproduction and developmental alterations in transformed plants. A chimaeric gene constructed by positioning the ipt coding region under the control of the hsp70 gene from Drosophila melanogaster allows heat-regulated expression in transgenic plant tissue. Heat-shock treatment of tobacco calli transgenic for the chimaeric hsipt gene increases the endogenous cytokinin concentration and enables these calli to grow on cytokinin-free medium. Transgenic plants regenerated from calli transformed with the hsipt gene and grown at normal temperature are phenotypically normal.

【 授权许可】

Unknown