Insertion of short hepatitis virus A amino acid sequences into poliovirus antigenic determinants results in viable progeny

FEBS Letters

Markova, S.V.³  Azhikina, T.L.³  Rostapshov, V.M.³  Chernov, I.P.³  Sverdlov, E.D.³  Teterina, N.L.¹  Kolesnikova, M.S.²  Agol, V.I.²  Tsarev, S.A.³  Romanova, L.I.²  Tolskaya, E.A.²  Gorbalenya, A.E.² 

[1] M.V. Lomonosov Moscow State University, Moscow, USSR;Institute of Poliomyelitis and Viral Encephalitides, USSR Academy of Medical Sciences, Moscow Region, USSR;M.M. Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow, USSR

关键词: Poliovirus;    Hepatits A virus;    Chimeric virus;    cDNA;    infectious;   

DOI  :  10.1016/0014-5793(89)81570-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In an infectious poliovirus cDNA construct, the determinant encoding antigenic epitope N-Agl (in a loop located between two β-strands in polypeptide VP1) was altered by site-directed mutagenesis, to be partially similar with the determinants for presumptive epitopes in polypeptides VP1 or VP3 of hepatitis A virus (HAV). The modified constructs proved to be infectious. However, another construct, in which the same locus encoded a ‘nonsense’ and a relatively hydrophobic amino acid sequence, exhibited no infectivity. These data showed the feasibility of the insertion of foreign sequences in a specific antigenically active locus of the poliovirus icosahedron, and suggest some limitations with respect to the sequences to be ‘transplanted’.

【 授权许可】

Unknown