Ras protein expression is developmentally regulated in embryonic chicken brain

FEBS Letters

Klinz, Franz-Josef¹ 

[1] Department of Neuropharmacology, Max-Planck-Institut für Psychiatrie, Am Klopferspitz 18a, D-8033 Planegg-Martinsried, FRG

关键词: Ras p21 protein;    Immunoblot analysis;    Membrane pool;    Microsomal pool;    Developmental regulation;    Rodent glial cell;    (Embryonic chicken brain);    E6;    embryonic day 6;    GFAP;    glial fibrillary acidic protein;    NF68;    neurofilament 68;    mAb;    monoclonal antibody;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(89)81564-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Immunoblot analysis using a panreactive monoclonal antibody directed against ras p21 proteins detects two differentially regulated ras pools in embryonic chicken brain: a membrane pool that is not changed and a microsomal pool that starts at a low level but is strongly increased from E6 to E16 chicken brain. In order to study the distribution of ras proteins in different cell types of the nervous system, immunoblot analysis was performed on total cell proteins. In contrast to histochemical data showing the absence or low levels of ras proteins in glial cells, comparable amounts of ras proteins were found in cell lysates from purified chicken sympathetic neurons, cultured rat Schwann cells and mouse brain astrocytes.

【 授权许可】

Unknown