Acidic and basic fibroblast growth factors similarly regulate the rate of biosynthesis of rat astroblast proteins

FEBS Letters

Labourdette, Gérard¹  Laeng, Pascal²  Sensenbrenner, Monique²  Loret, Camille² 

[1] INSERM U44, 5, rue Biaise Pascal, 67084 Strasbourg Cedex, France;Centre de Neurochimie du CNRS Strasbourg Cedex, France

关键词: Fibroblast growth factor;    Astroblast;    Two-dimensional gel electrophoresis;    Cell maturation;    CHAPS;    3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate;    FGF;    fibroblast growth factor;    GS;    glutamine synthetase;    [125I]dUrd;    [125I]iododeoxyuridine;    IEF;    isoelectric focusing;    LDS-PAGE;    lithium dodecylsulfate-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(89)81562-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Quiescent rat astroblasts in culture have been treated for various periods of time with acidic and basic fibroblast growth factors. Both factors elicited similar effects on the cell proliferation and glutamine synthetase activity. The rate of biosynthesis of the proteins analyzed on autoradiograms of polyacrylamide gels after two-dimensional electrophoresis was also similarly modulated by the two growth factors. These results suggest that the two fibroblast growth factors act through the same membrane receptors on rat astroblasts in culture.

【 授权许可】

Unknown