Elevation of apolipoprotein E in the CSF of cattle affected by BSE

FEBS Letters

Frutiger, Séverine¹  Wilkins, Marc R.²  Hochstrasser, Denis F.²  Hughes, Graham¹  Sanchez, Jean-Charles² 

[1] Medical Biochemistry Department, University Medical Center (CMU), Geneva University, CH-1211 Geneva 14, Switzerland;Clinical Chemistry Laboratory, Geneva University Hospital (HUG), 24 Micheli-du-Crest, CH-1211 Geneva 14, Switzerland

关键词: Bovine spongiform encephalopathy;    Creutzfeldt-Jakob disease;    Two-dimensional gel electrophoresis;    Apolipoprotein E;    Cerebrospinal fluid;   

DOI  :  10.1016/S0014-5793(97)01191-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cerebrospinal fluid (CSF) of patients suffering from Creutzfeldt-Jakob disease (CJD) display two unique polypeptide chains by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). In the absence of a well-defined ante-mortem diagnostic test for bovine spongiform encephalopathy (BSE), spinal fluid samples of eight normal cows and eight cows known to carry BSE by post-mortem histological analysis were investigated to verify if equivalent polypeptides were present. Proteins with similar migration to human CJD polypeptides were not detected. But surprisingly, a cluster of polypeptide spots that was faint or not detected in normal bovine CSF samples was found to be elevated or massively increased in BSE CSF samples (more than 10-fold increase). These elevated polypeptide chains were identified as apolipoprotein E.

【 授权许可】

Unknown