期刊论文详细信息
FEBS Letters | |
Purification of a DNA methyltransferase from Bacillus natto B3364 | |
Kim, Ella L.1  Maliuta, Staniclav S.1  | |
[1] Institute of Molecular Biology and Genetics, Zabolotny 150, 252143 Kiev, USSR | |
关键词: Restriction/modification; DNA methylation; Enzyme purification; Adenosyl-L-methionine; S-; (Bacillus natto); | |
DOI : 10.1016/0014-5793(89)81122-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
An S-adenosyl-L-methionine:DNA-methyltransferase, termed M · BnaI, was purified from Bacillus natto B3364 strain by successive column chromatography. The molecular weight determined by gel filtration was 37 kDa for M · BnaI. Analysis of methyltransferase by sodium dodecyl sulfate—polyacrylamide gel electrophoresis showed correspondence of the M · BnaI activity with one protein band at a molecular weight of 35 kDa. Sequencing of pUC19 DNA methylated with M · BnaI showed the cytosine-5 methylation in the BnaI recognition sequence GGAT↓CC at the position indicated by the arrow.
【 授权许可】
Unknown
【 预 览 】
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