Purification of a DNA methyltransferase from Bacillus natto B3364

FEBS Letters

Kim, Ella L.¹  Maliuta, Staniclav S.¹ 

[1] Institute of Molecular Biology and Genetics, Zabolotny 150, 252143 Kiev, USSR

关键词: Restriction/modification;    DNA methylation;    Enzyme purification;    Adenosyl-L-methionine;    S-;    (Bacillus natto);   

DOI  :  10.1016/0014-5793(89)81122-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An S-adenosyl-L-methionine:DNA-methyltransferase, termed M · BnaI, was purified from Bacillus natto B3364 strain by successive column chromatography. The molecular weight determined by gel filtration was 37 kDa for M · BnaI. Analysis of methyltransferase by sodium dodecyl sulfate—polyacrylamide gel electrophoresis showed correspondence of the M · BnaI activity with one protein band at a molecular weight of 35 kDa. Sequencing of pUC19 DNA methylated with M · BnaI showed the cytosine-5 methylation in the BnaI recognition sequence GGAT↓CC at the position indicated by the arrow.

【 授权许可】

Unknown