Direct identification of the fluoroaluminate and fluoroberyllate species responsible for inhibition of the mitochondrial F1‐ATPase

FEBS Letters

Vignais, Pierre V.¹  Dupuis, Alain¹  Issartel, Jean-Paul¹ 

[1] Laboratoire de Biochimie, LBIO DRF, Centre d'Etudes Nucléaires de Grenoble, 85X, 38041 Grenoble Cedex, France

关键词: Fluoride;    Beryllium;    Aluminum;    ATPase;    F1-;    Nucleotide analogue;    NDP nucleoside diphosphate;    NTP nucleoside triph.;    pF;    colog of the free fluoride concentration;    CDTA trans-1;    2 diaminocyclohexane-N;    N;    N′;    N′-tetraacetic acid;   

DOI  :  10.1016/0014-5793(89)81058-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the presence of ADP, fluoroaluminate and fluoroberyllate inhibit irreversibly the soluble mitochondrial F₁-ATPase. We report here direct evidence that this inhibition is related to the tight binding of [³H]ADP, beryllium and fluoride to the enzyme. In the case of beryllium-induced inhibition, the stoichiometry of bound species is 1 mol [³H]ADP, 1 mol beryllium and 2 or 3 mol fluoride depending on the initial fluoride concentration used, which indicates that both the combinations ADP₁,Be₁,F₃ and ADP₁,Be₁,F₃ are competent for inhibition. In the case of aluminium-induced inhibition, the binding stoichiometry of 4 mol fluoride per mol [³H]ADP favours the following combination of bound species ADP₁,Al₁,F₄. These results favour a model where fluorometals mimic phosphate and form an abortive complex with ADP in the catalytic site(s) of F₁.

【 授权许可】

Unknown