AVP receptors of mouse Leydig cells are regulated by LH and E2 and influenced by experimental cryptorchidism

FEBS Letters

Pointis, Georges¹  Tahri-Joutei, Abderrafih¹ 

[1] INSERM U. 166, Maternité Baudelocque, 123 Bld de Port-Royal, 75014 Paris, France

关键词: Vasopressin;    Receptor;    Testosterone;    Cryptorchidism;    (Leydig cell;    Mouse);    AVP;    Arg8-vasopressin;    OT;    oxytocin;    oLH;    ovine luteinizing hormone;    hCG;    human chorionic gonadotropin;    E2;    17β-estradiol;   

DOI  :  10.1016/0014-5793(89)81036-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Exposure of pubertal mouse Leydig cells for 24 h to increasing concentrations (1–100 ng/ml) of LH elicited a dose-dependent decrease in AVP receptor content. Maximal reduction (50%) was obtained at a dose of 10 ng/ml LH. A similar treatment applied to adult Leydig cells did not influence AVP receptor density. Treatment of adult Leydig cells for 24 h by E₂ (5–500 ng/ml) resulted in a dose-dependent increase in AVP receptor content. About 50% increase was achieved with 500 ng/ml E₂. AVP receptor content in pubertal Leydig cells was not modified irrespective of the concentration of E₂ tested. These changes in AVP receptor number were well correlated with the response of Leydig cells to AVP (10⁻⁶M) in terms of testosterone production. 2 weeks bilateral cryptorchidism resulted in reduction of testicular weight, circulating testosterone levels associated with a marked rise in Leydig cell AVP receptor density with no change of affinity. Testosterone production by Leydig cells from cryptorchid testes in response to AVP (10⁻⁶ M) or hCG (100 ng/ml) stimulation was reduced compared to that of control Leydig cells. This study provides new arguments supporting the concept that AVP could be involved in local regulation of testicular steroidogenesis.

【 授权许可】

Unknown