Chloride binding to photosystem II in the dark is in slow exchange

FEBS Letters

Brudvig, Gary W.¹  Shachar-Hill, Yair¹  Beck, Warren F.¹ 

[1] Dept of Chemistry, Yale University, New Haven, CT 06511, USA

关键词: Photosystem II;    Chloride;    NMR;    35Cl−   

DOI  :  10.1016/0014-5793(89)81035-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have studied the ³⁵Cl⁻ NMR line broadening in the presence of photosystem II (PS II) membranes from spinach in the dark. In the presence of NH₃ (which other work has shown to competitively inhibit chloride binding to PS II) we observed no decrease in ³⁵Cl⁻ linewidths. We conclude that binding of Cl⁻ to the O₂ evolving center in PS II in the dark (previously demonstrated by EPR) is in slow exchange on the NMR timescale. We assign the observed line broadening to interaction with non-specific binding sites and with free paramagnetics.

【 授权许可】

Unknown