Intracellular targetting signals of polymeric immunoglobulin receptors are highly conserved between species

FEBS Letters

Banting, George¹  Braghetta, Paola²  Luzio, J.Paul²  Brake, Brigitte¹  Stanley, Keith K.² 

[1] European Molecular Biology Laboratory, Postfach 10.2209, Meyerhofstrasse 1, D-6900 Heidelberg, FRG

关键词: Immunoglobulin receptor;    polymeric;    Trancytosis: Plasmid pUEX;    Expression cloning;    SDS—PAGE;    SDS-polyacrylamide gel electrophoresis;    SC;    secretory component;    pIgR;    polymeric immunoglobulin receptor;   

DOI  :  10.1016/0014-5793(89)81034-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A rat liver cDNA library, constructed in the plasmid expression vector pUEX, was immunoscreened using a rabbit polyclonal antiserum raised against rat liver Golgi membrane proteins. A sub-set of isolated clones were shown to encode the rat polymeric immunoglobulin receptor (pIgR). DNA sequence analysis of these clones provided the complete coding sequence of rat pIgR. Subsequent alignment of rat, rabbit and human predicted amino acid sequences demonstrated that the greatest degree of homology between the three pIgRs lies in their cytoplasmic tails; a region previously shown to be important for correct targetting and trancytosis of rabbit pIgR [(1984) Nature 308, 37–43].

【 授权许可】

Unknown