Interactions of formylmethionyl‐leucyl‐phenylalanine, adenosine, and phosphodiesterase inhibitors in human monocytes Effects on superoxide release, inositol phosphates and cAMP

FEBS Letters

Elliott, Keith R.F.¹  Leonard, Edward J.² 

[1] Department of Biochemistry and Molecular Biology, University of Manchester, Manchester M13 9PT, England;Immunopathology Section, National Cancer Institute, Frederick, Maryland, USA

关键词: Formylmethionyl-leucyl-phenylalanine;    Adenosine;    cyclic AMP;    Theophylline;    Superoxide release;    (Human monocyte);    fMLF;    formylmethionyl-leucyl-phenylalanine;    Gey-BSA;    Gey's balanced salt solution containing 2% bovine plasma albumin;    HBSS;    Hank's balanced salt solution;    PDE;    phosphodiesterase;    PIP2;    phosphatidylinositol 4;    5-bisphosphate;   

DOI  :  10.1016/0014-5793(89)81016-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cessation of the fMLF-induced burst of human monocyte superoxide release was associated with a rise in cAMP. This was not due to inhibition of phophodiesterase (PDE), the major form of which was the PDE IV isozyme. The action of burst inhibitors did not correlate with cAMP levels: Rolipram, a PDe IV inhibitor, increased cAMP 6-fold, with minimal effects on the burst; whereas theophylline increased cAMP less than 2-fold but decreased the burst to less than half. Although theophylline and the adenylate cyclase activator, adenosine, inhibited fMLF-induced superoxide release, they did not inhibit production of inositol phosphates. Thus, these studies on inhibition of superoxide release implicated neither cAMP nor inositol phosphates.

【 授权许可】

Unknown