The Na+‐motive respiration in Escherichia coli

FEBS Letters

Dibrov, P.A.¹  Avetisyan, A.V.¹  Sokolov, M.V.¹  Skulachev, V.P.¹ 

[1] Department of Bioenergetics, A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 119899, USSR

关键词: Na+-motive respiratory chain;    Protonophore;    Uncoupler;    (E. coli);    Δµ̃H and Δµ̃Na;    H+ and Na+ electrochemical potential differences;    respectively;    Δψ;    electric potential difference;    CCCP;    carbonyl cyanide m-chlorophenylhydrazone;    DEA;    diethylamine;    HQNO;    2-heptyl-4-hydroxyquinoline N-oxide;    TPP+;    tetraphenylphosphonium cation;   

DOI  :  10.1016/0014-5793(89)81000-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The protonophorous uncoupler carbonyl cyanide m-chlorophenylhydrazone was found to inhibit the growth rate of E. coli and maintenance of the membrane potential (Δψ) in intact bacterial cells. Both inhibitory effects of the protonophore were strongly decreased by Na⁺. A favourable action of Na⁺ could be observed only when cells were grown under conditions of low Δµ̃H, i.e. at alkaline pH (8.6) or in the presence of the photonophore in growth medium. It was abolished by addition of ΔpH-dissipating agents, i.e. the penetrating weak base diethylamine and the Na⁺/H⁺ antiporter monensin, to the incubation medium. In inside-out subcellular vesicles isolated from cells grown at alkaline pH, NADH oxidation was shown to be coupled to Na⁺ uptake which was markedly stimulated by protonophore and inhibited by cyanide and HQNO. It is concluded that the growth of E. coli at alkaline pH or in the presence of protonophore gives rise to the induction of a primary respiratory Na⁺ pump(s).

【 授权许可】

Unknown