Mutual inactivation of valinomycin and protonophores by complex formation in liposomal membranes

FEBS Letters

Krishnamoorthy, G.¹ 

[1] Chemical Physics Group, Tata Institute of Fundamental Research, Homi Bhabha Road, Bombay 400 005, India

关键词: Valinomycin;    Protonophore;    Ion transport inactivation;    Proton transport;    Temperature-induced pH jump;    SF6847;    3;    5-di(tert-butyl)-4-hydroxybenzylidenemalononitrile;    CCCP;    carbonyl cyanide m-chlorophenylhydrazone;    T-jump;    temperature jump;    pyranine;    8-hydroxy-1;    3;    6-pyrenetrisulfonic acid;   

DOI  :  10.1016/0014-5793(88)80416-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The simultaneous presence of a protonophore [3,5-di(tert-butyl)-4-hydroxybenzylidenemalononitrile or carbonyl cyanide m-chlorophenylhydrazone] and valinomycin in a liposome suspension results in time-dependent inactivation of ion transport by both the protonophore and valinomycin. Correlation of the inactivation with spectrophotometric observations on the formation of a complex between the protonophore and valinomycin strongly suggests that the complex observed has no (or very low) activity for the transport of either H⁺ or K⁺. The stoichiometry of valinomycin and the protonophore in the inactive complex is shown to be 1:1.

【 授权许可】

Unknown