Membrane‐binding sites for acyl carrier protein in Escherichia coli

FEBS Letters

Therisod, Helene¹  Bayan, Nicolas¹ 

[1] Laboratoire des Biomembranes, UA 1116, Université Paris Sud, Bât 433, 91405 Orsay Cedex, France

关键词: Acyl carrier protein;    Plasma membrane;    Scatchard analysis;    (E. coli);   

DOI  :  10.1016/0014-5793(89)80963-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report that membrane vesicles of Escherichia coli contain protein-binding sites for acyl carrier protein. Scatchard analysis of the binding indicates a dissociation constant around 0.35 μM and a maximum number of protein-binding sites around 50 pmol per mg of membrane protein. Binding is on the inner membrane while the outer membrane is devoid of binding sites. These results are consistent with the fact that some acyl carrier protein-dependent enzymes implicated in phospholipid- and membrane-derived oligosaccharide biosynthesis are localized in the cytoplasmic membrane.

【 授权许可】

Unknown