Substrate specificity of wheat embryo calcium‐dependent protein kinase

FEBS Letters

Morrice, Nick¹  Polya, Gideon M.¹  Wettenhall, Richard E.H.¹ 

[1] Department of Biochemistry, La Trobe University, Bundoora, Victoria 3083 Australia

关键词: Ca2+;    Protein kinase;    Histone H1;   

DOI  :  10.1016/0014-5793(89)80946-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Wheat embryo Ca²⁺ -dependent protein kinase (CDPK) phosphorylates a variety of synthetic peptides having a Basic-X-X-Ser sequence but peptides with a Basic-Basic-X-Ser(Thr) sequence are relatively poor substrates. Wheat germ CDPK phosphorylates a variety of proteins of which histone H1 and bovine serum albumin are among the better substrates. A single phosphorylated tryptic peptide was purified from bovine histone H1 phosphorylated by wheat embryo CDPK and subjected to Edman degradation yielding the sequence Gly⁹⁷-Thr-Gly-Ala-Ser-Gly-Ser(PO₄)-Phe-Lys¹⁰⁵. Ser¹⁰³ on bovine histone H1 is also the residue phosphorylated by rat brain protein kinase C.

【 授权许可】

Unknown