Activation of wheat embryo calcium‐regulated protein kinase by unsaturated fatty acids in the presence and absence of calcium

FEBS Letters

Polya, G.M.¹  Lucantoni, A.¹ 

[1] Department of Biochemistry, La Trobe University, Bundoora, Victoria 3083, Australia

关键词: Protein kinase;    Unsaturated fatty acid;    Ca2+;   

DOI  :  10.1016/0014-5793(87)80347-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Unsaturated fatty acids (oleic, linoleic and arachidonic acids) markedly activate extensively purified wheat embryo Ca²⁺-regulated protein kinase in the absence of Ca²⁺ or at very low free Ca²⁺ levels (<0.4 μM). While oleic and linoleic acids also activate in the presence of Ca²⁺ (10⁻⁶−10⁻⁴ M), arachidonic acid inhibits at free Ca²⁺ greater than 10⁻⁵ M. Phosphatidylserine does not substantially activate in the absence of Ca²⁺ or the presence of high Ca²⁺. Stearic and arachidic acids are less effective than the unsaturated fatty acids as activators in the absence of Ca²⁺. This type of plant protein kinase may be regulated in vivo independently of Ca²⁺ through release of unsaturated fatty acids.

【 授权许可】

Unknown