Cloning of a DNA region of Actinoplanes teichomyceticus conferring teicoplanin resistance

FEBS Letters

Lorenzetti, R.¹  Parenti, F.¹  Denaro, M.¹  Moroni, M.C.¹  Sosio, M.¹  Granozzi, C.¹ 

[1]Merrell Dow Research Institute, Lepetit Research Center, Via R. Lepetit 34, 21040 Gerenzano/VA, Italy

关键词: Teicoplanin;    A/40926;    Vancomycin;    Gene cloning;    Glycopeptide;    Antibiotics resistance;    (Actinoplanes teichomyceticus);   

DOI  :  10.1016/0014-5793(89)80940-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Teicoplanin is a glycopeptide antibiotic, produced by Actinoplanes teichomyceticus, active against Gram positive bacteria and recently introduced into clinical practice. It blocks cell wall biosynthesis by inhibiting peptidoglycan polymerization. The mechanism(s) of resistance of the producer strains of this class of antibiotics have not yet been characterized. We have constructed a genomic bank of A. teichomyceticus in Streptomyces lividans. A clone from this bank, pTR168, was able to confer resistance to teicoplanin on its sensitive host. The restriction map of plasmid pTR168 and the hybridization pattern to A. teichomyceticus DNA were determined; we have also studied the mechanism of this resistance which seems correlated with a reduced binding of the antibiotic to the cell wall.

【 授权许可】

Unknown