The narK gene product participates in nitrate transport induced in Escherichia coli nitrate‐respiring cells

FEBS Letters

Noji, Sumihare²  Taniguchi, Shigehiko²  Saito, Taiichi¹  Nohno, Tsutomu¹ 

[1] Department of Pharmacology, Kawasaki Medical School, 577 Matsushima, Kurashiki City 701-01, Japan;Department of Biochemistry, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama City 700 Japan

关键词: Nitrate transport;    Gene;    narK;    Nitrate respiration;    (E. coli);   

DOI  :  10.1016/0014-5793(89)80906-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The nucleotide sequence of the Escherichia coli narK gene, which is located in the upstream region of the narCHJI operon, was determined. The narK gene encodes a very hydrophobic protein with 463 amino acid residues (M _r 49 693). A narK deletion mutant, under conditions for the induction of nitrate respiration, was unable to perform nitrate transport. Loss of transport activity was recovered by transforming the mutant with a narK ⁺ plasmid. Thus, we conclude that the narK gene encodes a transmembrane protein participating in nitrate transport. In the narK promoter region, we defined a unique sequence that we designate as a ‘nitrate box’, functioning as a putative NarL-binding site, in addition to the consensus sequence of the ‘anaero-box’.

【 授权许可】

Unknown