Substrate structural requirements of Schizosaccharomyces pombe RNase P

FEBS Letters

Drainas, Denis¹  Zimmerly, Steven¹  Söll, Dieter¹  Willis, Ian¹ 

[1] Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511, USA

关键词: RNase P;    tRNA;    Substrate recognition;    (Schizosaccharomyces pombe);   

DOI  :  10.1016/0014-5793(89)81433-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

RNase P from Schizosaccharomyces pombe has been purified over 2000-fold. The apparent K _m for two S. pombe tRNA precursors derived from the supS1 and sup3-e tRNA^Ser genes is 20 nM; the apparent V _max is 2.5 nM/min (supS1) and 1.1 nM/min (sup3-e). Processing studies with precursors of other mutants show that the structures of the acceptor stem and anticodon/intron loop of tRNA are crucial for S. pombe RNase P action.

【 授权许可】

Unknown