Retinoic acid enhances VIP receptor expression and responsiveness in human neuroblastoma cell, SH‐SY5Y

FEBS Letters

Waschek, James A.¹  Duan, Dah-Shuhn²  Sadée, Wolfgang²  Muller, Jean-Marc¹ 

[1] Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, MD 20892, USA;School of Pharmacy, University of California, San Francisco, CA 94143-0446, USA

关键词: Vasoactive intestinal peptide;    Vasoactive intestinal peptide receptor;    cyclic AMP stimulation;    Retinoic acid;    Neuroblastoma differentiation;    (SH-SY5Y human neuroblastoma cell);   

DOI  :  10.1016/0014-5793(89)80806-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Retinoic acid (RA) induces partial differentiation of neuroblastoma (NB) cells in vitro. In the human NB line, SH-SY5Y (a neuroblastic subclone of SK-N-SH), RA was previously shown to enhance the stimulatory (PGE₁) and inhibitory (opioid) regulation of adenylyl cyclase. Since these cells are also sensitive to cAMP stimulation by vasoactive intestinal peptide (VIP), we have tested the effects of RA on VIP receptor expression and function. Pretreatment of SH-SY5Y cells with 10 μM RA over 6 days dramatically increased VIP receptor number from ∼3 000 to ∼70 000 sites per cell and enhanced threefold the cAMP accumulation after external VIP addition, while VIP immunoreactive content in the cells increased 2–3-fold. In the light of the recently proposed autocrine function of VIP in this cell lineage, the strong enhancement of the VIP system may contribute to the differentiation effects of RA.

【 授权许可】

Unknown