Presence of Vasoactive Intestinal Peptide Receptor in the Hen Hypothalamus

Endocrine Journal

MICHIHARU KAMIYOSHI²  KATUHIDE TANAKA¹  KENJI ICHINOE¹  MITSUO KAWASHIMA²  SYLVIA MAST GONZALES¹ 

[1] The Department of Zootechnical Science, Tokyo University of Agriculture;The Department of Animal Science and Technology, Gifu University

关键词: Vasoactive intestinal peptide receptor;    Radioligand assay;    Scatchard analyses;    Hypothalamus;    Laying hen;   

DOI  :  10.1507/endocrj.42.179

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(31)Cited-By(3)Radioligand assays of the membrane fraction of hen hypothalamic tissues involving the preoptic (HPOA) or median eminence (HMEA) areas revealed the presence of a specific binding component to chicken vasoactive intestinal peptide (cVIP) having properties of a receptor. The equilibrium dissociation constant (Kd) was 0.70±0.07nM (Mean±SEM; N=5) in HPOA and 1.02±0.15nM (N=5) in HMEA as estimated by Scatchard analysis of saturation studies, and was 0.91±0.11nM (N=3) (HPOA) and 1.25 ±0.09nM (N=3) (HMEA) as determined by a kinetic analysis. The maximum binding capacity (Bmax) obtained by Scatchard analysis was 167±19fmol/mg protein (N=5) (HPOA) and 133±17fmol/mg protein (N=5) (HMEA). The Kd and Bmax values obtained by Scatchard analysis were similar in the two areas of the hypothalamus and in both laying and nonlaying hens. Administration of cVIP in vivo caused a decrease in specific cVIP binding. These results suggest the presence of a VIP receptor in the hen hypothalamus.

【 授权许可】

Unknown