FEBS Letters | |
Histamine and serotonin inhibit induction of ornithine decarboxylase by ornithine in perifused Ehrlich ascites tumour cells | |
de Castro, I.Núñez1  Matés, J.M.1  García-Caballero, M.2  Sánchez-Jiménez, F.M.1  | |
[1] Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Málaga, 29071, Málaga, Spain;Cátedra de Cirugía, Universidad de Málaga, 29071, Málaga, Spain | |
关键词: Ornithine decarboxylase; Histamine; Serotonin; Perifusion; | |
DOI : 10.1016/0014-5793(89)80733-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Ornithine induced more than 36-fold the ornithine decarboxylase activity in confined Ehrlich ascites tumour cells after 3.5 h of continuous perifusion with 0.5 mM ornithine; arginine and glutamine also induced the activity 3- and 4-fold, respectively. The addition of cycloheximide or actinomycin D antibiotics to the perifusion medium confirmed that the regulation of the enzyme synthesis takes place at the level of translation. Perifusion in the presence of 0.5. mM ornithine and 55, 25, and 10 μM histamine suppressed the induction by 91, 53, and 35%, respectively. Similar results were obtained in the presence of serotonin. Histidine also showed inhibitory effect but 5 mM histidine was required to produce 21% inhibition; other basic amino acids were ineffective.
【 授权许可】
Unknown
【 预 览 】
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