Cytochrome o (bo) is a proton pump in Paracoccus denitrificans and Escherichia coli

FEBS Letters

Finel, Moshe¹  Puustinen, Anne¹  Virkki, Marika¹  Wikström, Mårten¹ 

[1] Department of Medical Chemistry, University of Helsinki, Siltavuorenpenger 10, SF-00170 Helsinki, Finland

关键词: Cytochrome o complex;    Cytochrome-c oxidase;    Electron transfer;    Energy coupling;    Proton translocation;    FCCP;    carbonyl cyanide p-trifluoromethoxyphenylhydrazone;    MX;    myxothiazol;    UQ-1 ubiquinonc-1;    QH2;    ubiquinol;   

DOI  :  10.1016/0014-5793(89)80616-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Spheroplasts from aerobically grown wild-type Paracoccus denitrificans cells respire with succinate despite specific inhibition of the cytochrome bc ₁ complex by myxothiazol. Coupled to this activity, which involves only b-type cytochromes, there is translocation of 1.5–1.9 H⁺/e⁻ across the cytoplasmic membrane. Similar H⁺ translocation ratios are observed during oxidation of ubiquinol in spheroplasts from aerobically grown mutants of Paracoccus lacking cytochrome c oxidase, or deficient in cytochrome c, as well as in a strain of E. coli from which cytochrome d was deleted. These observations show that the cytochrome o complex is a proton pump much like cytochrome aa ₃ to which it is structurally related.

【 授权许可】

Unknown