Oscillating enzyme‐bound NADH in glycolysis

FEBS Letters

Müller, S.C.¹  Yuan, Z.¹  Hess, B.¹ 

[1] Max-Planck-Institut für Ernährungsphysiologie, Rheinlanddamm 201, 4600 Dortmund 1, FRG

关键词: Glycolysis;    Oscillation;    NADH;    enzyme-bound;    Action spectrum;    Dialysis;   

DOI  :  10.1016/0014-5793(89)80615-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In glycolyzing cell-free cytoplasmic medium extracted from yeast Saccharomyces cerevisiae, the action spectrum of oscillation has an absorption maximum around 335 nm, nearly coinciding with that of the yeast alcohol dehydrogenase (ADH)-NADH complex due to its bound NADH. Our approximate calculations based on the amount of this enzyme and coenzyme NADH present in the extract suggest that the ADH-NADH complex alone can account for 90% or more of the total absorbance change.

【 授权许可】

Unknown