The role of charge neutralization and cooperative binding of linker histone in. the higher‐order structure of chromatin

FEBS Letters

Watanabe, Fumiyuki¹ 

[1] Institut für Zellbiologie, ETH Hönggerberg, Zürich, CH-8093, Switzerland

关键词: Chromatin;    Histone H1;    Cooperativity;    DNA-protein interaction;    Fluorescence;   

DOI  :  10.1016/0014-5793(89)80612-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The binding mode and stoichiometry of interaction between soluble rat liver chromatin and histone H1 (H1) were studied. H1 binding to chromatin is cooperative. Chromatin accepts 3.6 molecules of H1/nucleosome at 0 M salt, close to the required ratio for neutralization of 90% of the charges on the phosphate groups of chromatin (4.0 H1 molecules/nucleosome). The proposal is put forward that critical charge neutralization (90%) has a significant influence on the irregular appearance of chromatin.

【 授权许可】

Unknown