期刊论文详细信息
FEBS Letters | |
The role of charge neutralization and cooperative binding of linker histone in. the higher‐order structure of chromatin | |
Watanabe, Fumiyuki1  | |
[1] Institut für Zellbiologie, ETH Hönggerberg, Zürich, CH-8093, Switzerland | |
关键词: Chromatin; Histone H1; Cooperativity; DNA-protein interaction; Fluorescence; | |
DOI : 10.1016/0014-5793(89)80612-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The binding mode and stoichiometry of interaction between soluble rat liver chromatin and histone H1 (H1) were studied. H1 binding to chromatin is cooperative. Chromatin accepts 3.6 molecules of H1/nucleosome at 0 M salt, close to the required ratio for neutralization of 90% of the charges on the phosphate groups of chromatin (4.0 H1 molecules/nucleosome). The proposal is put forward that critical charge neutralization (90%) has a significant influence on the irregular appearance of chromatin.
【 授权许可】
Unknown
【 预 览 】
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RO201912020292053ZK.pdf | 242KB | download |