Subtype‐specific increase in G‐protein α‐subunit mRNA by interleukin 1β

FEBS Letters

Brock, Tommy A.²  Lee, Richard T.¹  Seidman, J.G.³  Tolman, Cynthia¹  Bloch, Kenneth D.³  Neer, Eva J.¹ 

[1] Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA;Vascular Research Division, Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA;Department of Genetics, Harvard Medical School, Boston, MA 02115, USA

关键词: Interleukin 1;    Guanine nucleotide regulatory protein;    mRNA;    (Endothelial cell);   

DOI  :  10.1016/0014-5793(89)80610-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The guanine nucleotide regulatory proteins (G-proteins) which are substrates for ADP-ribosylation by pertussis toxin (α_i−1, α_i−2, α_i−3 and α₀) transduce a variety of hormonal signals. Endothelial cells express mRNA for three α_i subtypes although the level of α_i−1 mRNA is very low. Interleukin 1β (IL1β), a pleiotropic inflammatory mediator which stimulates a complex series of responses in human endothelial cells leading to increased coagulation and platelet adhesion, increases expression of one subtype of α_i (α_i−2) mRNA in human endothelial cells as determined by Northern blot analysis without affecting the level of mRNA for other α-subunits. These studies show that mRNA levels for α_i subtypes are independently regulated, suggesting that there may be subtype specificity in the cell's requirements for the G_i class of signal-transducing proteins.

【 授权许可】

Unknown