Detection of multiple forms of Giα in HL60 cells

FEBS Letters

Spiegel, Allen¹  Brann, Mark¹  Eide, Brock¹  Murphy, Philip M.³  Gierschik, Peter²  Goldsmith, Paul¹  Malech, Harry L.³ 

[1] Molecular Pathophysiology Section, Metabolic Diseases Branch, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA;Pharmacology Institute, Heidelberg University, FRG;Bacterial Diseases Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA

关键词: Guanine nucleotide regulatory protein;    mRNA;    Differentiation;    Signal transduction;    Pertussis toxin;    (HL60 cell);   

DOI  :  10.1016/0014-5793(87)80356-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Comparison of cDNA sequences from multiple sources predicts a genus of highly homologous but structurally distinct G protein α-subunits, designated as G_iα, that may include the α-subunit of the functionally defined adenylate cyclase inhibitory G protein. Using specific oligonucleotide probes on Northern blots, we show that G_iα−2 and G_iα−3, but not G_iα−1, are expressed in HL60 cells. Antisera raised against synthetic peptides from regions predicted to be conserved (AS6) and divergent (LE3) among G_iα subtypes bind to a 40 kDa protein in Western blots of HL60 membranes. AS6 identifies an additional protein at 41 kDa. Thus, Northern blot and immunoblot results show that at least two G_iα subtypes, a 40 kDa protein likely to correspond to G_iα−2 and a 41 kDa protein possibly representing G_iα−3, may be expressed in a single cell type.

【 授权许可】

Unknown