FEBS Letters | |
[14C]Acetate incorporation into glycerolipids from cauliflower proplastids and sycamore amyloplasts | |
Dorne, Albert-Jean1  Alban, Claude1  Joyard, Jacques1  Douce, Roland1  | |
[1] Laboratoire de Physiologie Cellulaire Végétale, URA CNRS no. 576, DRF/LBIO, Centre d'Etudes Nucléaires et Université Joseph Fourier de Grenoble, 85 X, F-38041 Grenoble Cedex, France | |
关键词: Proplastid; Amyloplast; Glycerolipid; Monogalactosyldiacylglycerol; Acetate incorporation; Fatty acid desaturation; MGDG; monogalactosyldiacylglycerol; DGDG; digalactosyldiacylglycerol; LPA; lysophosphatidic acid; PA; phosphatidic acid; DG; diacylglycerol; UFA; unesterified fatty acids; TLC; thin-layer chromatography; | |
DOI : 10.1016/0014-5793(89)80023-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Formation of glycerolipids from [14C]acetate within proplastids from cauliflower buds and amyloplasts from sycamore cells was compared. In cauliflower proplastids, when sn-glycerol 3-phosphate was present, most of the radioactivity was recovered as diacylglycerol, which was converted into MGDG after addition of UDP-galactose. The MGDG molecular species synthesized in these conditions was C18:1/C16:0 and was rapidly desaturated into C18:3/C16:0. In sycamore amyloplasts, phosphatidic acid, and not diacylglycerol, was the major compound to be synthesized in the presence of sn-glycerol 3-phosphate, therefore, MGDG synthesis from [14C]acetate was not possible in sycamore amyloplasts. We conclude that non-green plastids almost behave like chloroplasts from C16:3 or C18:3 plants as far as lipid biosynthesis is concerned.
【 授权许可】
Unknown
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