[14C]Acetate incorporation into glycerolipids from cauliflower proplastids and sycamore amyloplasts

FEBS Letters

Dorne, Albert-Jean¹  Alban, Claude¹  Joyard, Jacques¹  Douce, Roland¹ 

[1] Laboratoire de Physiologie Cellulaire Végétale, URA CNRS no. 576, DRF/LBIO, Centre d'Etudes Nucléaires et Université Joseph Fourier de Grenoble, 85 X, F-38041 Grenoble Cedex, France

关键词: Proplastid;    Amyloplast;    Glycerolipid;    Monogalactosyldiacylglycerol;    Acetate incorporation;    Fatty acid desaturation;    MGDG;    monogalactosyldiacylglycerol;    DGDG;    digalactosyldiacylglycerol;    LPA;    lysophosphatidic acid;    PA;    phosphatidic acid;    DG;    diacylglycerol;    UFA;    unesterified fatty acids;    TLC;    thin-layer chromatography;   

DOI  :  10.1016/0014-5793(89)80023-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Formation of glycerolipids from [¹⁴C]acetate within proplastids from cauliflower buds and amyloplasts from sycamore cells was compared. In cauliflower proplastids, when sn-glycerol 3-phosphate was present, most of the radioactivity was recovered as diacylglycerol, which was converted into MGDG after addition of UDP-galactose. The MGDG molecular species synthesized in these conditions was C18:1/C16:0 and was rapidly desaturated into C18:3/C16:0. In sycamore amyloplasts, phosphatidic acid, and not diacylglycerol, was the major compound to be synthesized in the presence of sn-glycerol 3-phosphate, therefore, MGDG synthesis from [¹⁴C]acetate was not possible in sycamore amyloplasts. We conclude that non-green plastids almost behave like chloroplasts from C16:3 or C18:3 plants as far as lipid biosynthesis is concerned.

【 授权许可】

Unknown