Insulin antagonises the growth hormone‐mediated increase in the activity of phosphatidate phosphohydrolase in isolated rat hepatocytes

FEBS Letters

Bracken, Paul¹  Brindley, David N.¹  Fears, Robin²  Pittner, Richard A.¹ 

[1] Department of Biochemistry, University of Nottingham Medical School, Queen's Medical Centre, Nottingham NG7 2UH England;Beecham Pharmaceuticals, Biosciences Research Centre, Great Burgh, Yew Tree Bottom Road, Epsom KT18 5XQ, England

关键词: Glycerolipid Growth hormone Insulin L-α-Phosphatidate phosphohydrolase;   

DOI  :  10.1016/0014-5793(86)80663-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rat hepatocytes were incubated in monolayer culture, under serum-free conditions for 8 h. Rat growth hormone (up to 100 nM) increased the activity of phosphatidate phosphohydrolase by up to 41%. Insulin (500 pM or 35 nM), cycloheximide or actinomycin D reversed this effect. The ability of growth hormone to modify the effects of insulin is discussed in relation to the control of the phosphohydrolase activity and glycerolipid synthesis.

【 授权许可】

Unknown