ATP synthesis and pyrophosphate‐driven proton transport in tonoplast‐enriched vesicles isolated from Catharanthus roseus

FEBS Letters

Dupaix, Alain¹  Arrio, Bernard¹  Johannin, Georges¹ 

[1] URA 1116 du CNRS, Bioénergétique Membranaire, Bât. 433, Université de Paris-Sud, Centre d'Orsay, 91405 Orsay Cedex, France

关键词: Tonoplast vesicle;    Pyrophosphatase;    ATP synthesis;    (Catharanthus roseus);    Hepes;    4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid;    BTP;    bis-tris-propane;    Ap5A;    P1;    P5-di(adenosine-5′) pentaphosphate;    Pi;    inorganic phosphate;    PPi;    inorganic pyrophosphate;    PPase;    inorganic pyrophosphatase;   

DOI  :  10.1016/0014-5793(89)80005-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the presence of PP_i as an energy source, the tonoplast-bound inorganic pyrophosphatase from Catharanthus roseus cells is able to create a proton-gradient which can drive the synthesis of ATP from ADP and P_i. ATP synthesis is linked to the pH-gradient dissipation as monitored by the recovery of the fluorescence intensity of quinacrine and by the amount of synthesized ATP measured by the bioluminescent luciferin/luciferase assay. Proton gradient and ATP synthesis were suppressed by the protonic ionophore gramicidin D.

【 授权许可】

Unknown