Inorganic pyrophosphate‐driven ATP‐synthesis in liposomes containing membrane‐bound inorganic pyrophosphatase and F0‐F1 complex from Rhodospirillum rubrum

FEBS Letters

Nyrén, Pål¹  Baltscheffsky, Margareta¹ 

[1] Department of Biochemistry, Arrhenius Laboratory, University of Stockholm, S-106 91 Stockholm, Sweden

关键词: Pyrophosphatase;    Liposome;    Reconstitution;    ATPase;    ATP synthesis;    Proton pump;    DCCD;    N;    N′-dicyclohexylcarbodiimide;    DTE;    dithioerythritol;    FCCP;    carbonyl cyanide p-trifluoromethoxyphenylhydrazone;    IDP;    imidodiphosphate;    MDP;    methylenediphosphonate;   

DOI  :  10.1016/0014-5793(83)80223-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

PP₁ driven ATP synthesis has been reconstituted in a liposomal system containing the membrane-bound energy-linked PP₁ase and coupling factor complex, both highly purified from Rhodospirillum rubrum. This energy converting model system was made by mixing both enzyme preparations with an aqueous suspension of sonicated soybean phospholipids and subjecting to a freeze—thaw procedure. In the presence of ADP, Mg²⁺, P₁ and PP₁ the system catalyzed phosphorylation by up to 25 nmol ATP formed X mg protein⁻¹ X min⁻¹, at 20°C, which was sensitive to uncouplers and inhibitors of phosphorylation such as oligomycin, efrapeptin and N,N′-dicyclohexylcarbodiimide.

【 授权许可】

Unknown