Characterisation of enkephalinase (EC 3.4.24.11) activity on various leukemic cells expressing the common acute lymphocytic leukemia antigen (CALLA)

FEBS Letters

Gros, Claude³  Arock, Michel¹  Schwartz, Jean-Charles³  Devillier, Philippe²  Guillosson, Jean-Jacques¹ 

[1] Laboratoire d'Hématologie, Faculté de Pharmacie, 4 Avenue de l'Observatoire, 75006 Paris, France;Laboratoire Bioprojet, 30 rue des Francs Bourgeois, 75003 Paris, France;Unité de Neurobiologie et Pharmacologie (U. 109) de l'INSERM, Centre Paul Broca, 2ter rue d'Alésia, 75014 Paris, France

关键词: Common acute lymphocytic leukemia antigen;    Enkephalinase;    Neutral endopeptidase;    (REH cell line;    Pre-B leukemic cell);   

DOI  :  10.1016/0014-5793(89)80445-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The deduced amino acid sequences of CALLA, a cell surface marker of human acute lymphocytic leukemia, and human enkephalinase (neutral endopeptidase, EC 3.4.24.11) were recently reported to be almost identical. We show that membranes of CALLA⁺ cells of the REH lymphoblastic cell line as well as blast cells derived from the blood or bone marrow of patients with acute lymphocytic leukemia display high enkephalinase activity. This activity was abrogated by several enkephalinase inhibitors at concentrations closely similar to those required to inhibit pure human enkephalinase. However, these compounds did not significantly modify the rate of REH cell proliferation in vitro. Hence, the functional role, if any, of the high peptidase activity in lymphoblastic cells remains to be established.

【 授权许可】

Unknown