Molecular cloning and amino acid sequence of human enkephalinase (neutral endopeptidase)

FEBS Letters

Seeburg, Peter H.¹  Malfroy, Bernard³  Schofield, Peter R.¹  Kuang, Wun-Jing²  Mason, Anthony J.¹ 

[1] Developmental Biology, Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, CA 94080, USA;Molecular Biology, Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, CA 94080, USA;Department of Pharmacological Sciences, Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, CA 94080, USA

关键词: Enkephalinase;    Neutral endopeptidase;    Metallo peptidase;    cDNA cloning;    (Human);   

DOI  :  10.1016/0014-5793(88)80828-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated a cDNA clone encoding human enkephalinase (neutral endopeptidase, EC 3.4.24.11) in a λgt10 library from human placenta, and present the complete 742 amino acid sequence of human enkephalinase. The human enzyme displays a high homology with rat and rabbit enkephalinase. Like the rat and rabbit enzyme, human enkephalinase contains a single N-terminal transmembrane region and is likely to be inserted through cell membranes with the majority of protein, including its carboxy-terminus, located extracellularly.

【 授权许可】

Unknown