Zinc increases phorbol ester receptors in intact B‐cells, neutrophil polymorphs and platelets

FEBS Letters

Whitehouse, M.W.²  Zalewski, P.D.²  Forbes, I.J.²  Giannakis, C.²  Hurst, N.P.¹ 

[1] Rheumatology Unit, Queen Elizabeth Hospital, Woodville, SA 5081, Australia;Department of Medicine, University of Adelaide, Adelaide, Australia

关键词: Zinc;    Protein kinase C;    (B-cell;    Neutrophil;    Platelet);    CLL;    chronic lymphatic leukaemia;    PDBu;    phorbol dibutyrate;    PKC;    protein kinase C;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/0014-5793(89)81388-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the presence of pyrithione, which was used as a Zn²⁺ ionophore, Zn²⁺ (10–100 μM) increased phorbol ester binding by intact B-CLL cells in a dose-dependent fashion. Zn pyrithione increased 2-fold the number of phorbol ester receptors in B-cells (0.74 to 1.4 pmol/10⁶ cells), neutrophil polymorphs (0.2 to 0.51 pmol/10⁶ cells) and platelets (91 to 209 pmol/10¹⁰ cells). Fractionation of cells after treatment with Zn pyrithione showed that increased binding of PDBu occurred in the particulate fraction of cells and this was accompanied by loss of phorbol ester receptors from the cytosol. These data are compatible with a role for Zn in the subcellular distribution and activation of protein kinase C.

【 授权许可】

Unknown