Phosphoinositide hydrolysis in permeabilized SH‐SY5Y human neuroblastoma cells is inhibited by mastoparan

FEBS Letters

Nahorski, Stefan R.¹  Wojcikiewicz, Richard J.H.¹ 

[1] Department of Pharmacology and Therapeutics, University of Leicester, PO Box 138, Medical Sciences Building, University Road, Leicester LE1 9NH, England

关键词: Mastoparan;    Phosphoinositide hydrolysis;    G-protein;    Muscarinic receptor;    (SH-SY5Y cell);    GTP[S];    guanosine 5′-[γ-thio]triphosphate;    G-protein;    guanine nucleotide-binding regulatory protein;    PIP2;    phosphatidylinositol 4;    5-bisphosphate;    IP(s);    inositol phosphate(s);   

DOI  :  10.1016/0014-5793(89)81366-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of mastoparan on phospholipase C-catalysed phosphoinositide hydrolysis were examined in [³H]inositol-labelled human neuroblastoma SH-SY5Y cells. [³H]Inositol phosphate formation in intact cells was not altered by 20 μM mastoparan. In contrast, [³H]inositol phosphate formation in electrically permeabilized cells stimulated with guanosine 5′[γ-thio]triphosphate and/or carbachol was inhibited by mastoparan with half-maximal effects at approx. 3 μM. The peptide was much less effective in inhibiting stimulatory effects of Ca²⁺. Similar but less potent inhibitory effects were observed with the cations, neomycin and spermine, indicating that direct interaction of mastoparan with polyphosphoinositides might account for its inhibitory effects on inositol phosphate formation.

【 授权许可】

Unknown