The yeast pyruvate kinase gene does not contain a string of non‐ preferred codons: Revised nucleotide sequence

FEBS Letters

McNally, Teresa¹  Fothergill-Gilmore, Linda A.¹  Brown, Alistair J.P.²  Purvis, Ian J.² 

[1] Department of Biochemistry University of Edinburgh, George Square, Edinburgh EH8 9XD Scotland;Institute of Genetics, University of Glasgow, Church Street, Glasgow G11 5JS, Scotland

关键词: Pyruvate kinase gene;    Nucleotide sequence;    Translational control;    Non-preferred codon;    (Saccharomyces cerevisiae);   

DOI  :  10.1016/0014-5793(89)81359-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The sequence of the gene encoding pyruvate kinase from Saccharomyces cerevisiae was re-determined because of failures with oligonucleotide-directed mutagenesis experiments involving a region thought to contain a string of five contiguous non-preferred codons. This region was found to be difficult to sequence and was shown to have three extra bases when compared with the published sequence [(1983) J. Biol. Chem. 258, 2193–2201]. The revised sequence demonstrates that the yeast pyruvate kinase gene does not have a cluster of non-preferred codons, and that it therefore is not an example of the class of genes which possibly exhibit translational control by the presence of non-preferred codons.

【 授权许可】

Unknown