Pyrophosphate analogues in pyrophosphorolysis reaction catalyzed by DNA polymerases

FEBS Letters

Rozovskaya, Tatyana²  Chidgeavadze, Zurab¹  Krayevsky, Alexander²  Beabealashvilli, Robert¹  Tarussova, Natalya²  Atrazhev, Alexey²  Kukhanova, Marina²  Minassian, Shahnara² 

[1] National Cardiology Research Center, USSR Academy of Medical Sciences, 3rd Cherepkovskaya 15A, Moscow 121522, USSR;V.A. Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Vavilov str. 32, Moscow 117984, USSR

关键词: Pyrophosphorolysis;    DNA polymerase;    Pyrophosphate analog;   

DOI  :  10.1016/0014-5793(89)81354-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It is demonstrated here that rat liver DNA polymerase β catalyzes the pyrophosphorolysis reaction with pyrophosphate (PP_i) and its analogues. The substrate specificity of the PP_i-binding site of several DNA polymerases was investigated. It was discovered that the ability of DNA polymerases to utilize PP_i analogues instead of PP_i in the pyrophosphorolysis reaction was markedly restricted. Only imidodiphosphate and methylenediphosphonate were demonstrated as participating in this process. Oxodiphosphonate and phosphonoformate inhibited DNA synthesis, but probably not via the interaction with the PP_i-binding site of DNA polymerases.

【 授权许可】

Unknown