3'‐Fluoro‐2',3'‐dideoxyribonucleoside 5'‐triphosphates: terminators of DNA synthesis

FEBS Letters

Scamrov, Andrei V.¹  Zaitseva, Galina V.³  Kvasyuk, Eugenii I.³  Beabealashvilli, Robert Sh.¹  Chidgeavadze, Zurab G.¹  Langen, Peter²  Kowollik, Gotthard²  Mikhailopulo, Igor A.³ 

[1] National Cardiology Research Center of the USSR Academy of Medical Sciences, Moscow 121552, U.S.S.R.;Central Institute of Molecular Biology of the G D R Academy of Sciences, 1115 Berlin-Buch, GDR;Institute of Bioorganic Chemistry, Byelorussian SSR Academy of Sciences, Minsk 220600, USSR

关键词: 3'-Fluoro-2';    3'-dideoxyribonucleoside 5'-triphosphate;    Terminator;    DNA synthesis;    DNA polymerase;    Terminal deoxyribonucleotidyl transferase;    dNTP(3'F);    3'-fluoro-2';    3'-dideoxyribonucleoside 5'-triphosphates with the bases adenine [dATP(3'F)l;    guanine [dGTP(3'F)];    thymine [dTTP-(3'F)] and cytosine [dCTP(3 'F)];    dNMP(3'F);    3'-fluoro-2';    3'-dideoxyribonucleoside 5'-monophosphates with the same base nature;    dThd(3'F);    dCyt(3'F);    dAdo(3'F) and dGua(3'F);    3'-fluoro-2';    3'-dideoxynucleoside;    ddNTP;    2';    3'-dideoxynucleoside 5'-triphosphates;    dNTP(3 'NH2);    3'-amino-2';    3'-dideoxynucleoside 5'-triphosphates;    rNTP;    ribonucleoside 5'-triphosphates;   

DOI  :  10.1016/0014-5793(85)80792-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It is shown that dNTP(3'F) are terminators of DNA synthesis and may serve as very effective tools for DNA sequencing with E.coli DNA polymerase I and AMV reverse transcriptase. The dNTP(3'F) are found to be chain terminator substrates for calf thymus terminal deoxyribonucleotidyl transferase but not for calf thymus DNA polymerase α. The optimal dNTP(3'F) concentration for DNA sequencing by DNA polymerase I is found to be an order of magnitude lower than that of ddNTPs. dNTP(3'F) produce a more clear sequence pattern than do ddNTPs.

【 授权许可】

Unknown