A model for the dimeric molecular structure of phytochrome based on small‐angle X‐ray scattering

FEBS Letters

Tokunaga, Fumio³  Tokutomi, Satoru⁴  Nakasako, Masayoshi³  Furuya, Masaki²  Kataoka, Mikio³  Sakai, Jun²  Yamamoto, Kotaro T.⁴  Wada, Masamitsu¹ 

[1] Department of Biology, Faculty of Science, Tokyo Metropolitan University, Tokyo 158, Japan;Plant Biological Regulation Laboratory, Frontier Research Programs, RIKEN Institute, Wako City 351-01 Japan;Department of Physics, Faculty of Science, Tohoku University, Sendai 980, Japan;Division of Biological Regulation, National Institute for Basic Biology, Okazaki 444, Japan

关键词: Phytochrome;    Small-angle X-ray scattering;    Molecular structure;    Domain structure;    kDa;    kilodaltons;    Rg;    radius of gyration;    SAXS;    small-angle X-ray scattering;   

DOI  :  10.1016/0014-5793(89)81257-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The dimeric molecular structure of pea phytochrome (monomer molecular mass 114 kDa) in the red-light-absorbing form was studied with reference to the structures of its molecular domains in a monomer (an N-terminal 59-kDa chromophoric domain and a C-terminal 55-kDa nonchromophoric domain) using small-angle X-ray scattering, and a ‘four-leaved shape’ model is proposed. The propriety of the model was confirmed by images of the molecule obtained by rotary shadowing electron microscopy.

【 授权许可】

Unknown