Monitoring the transport and phosphorylation of 2‐deoxy‐D‐glucose in tumor cells in vivo and in vitro by 13C nuclear magnetic resonance spectroscopy

FEBS Letters

Lyon, Robbe C.¹  Cohen, Jack S.¹  Kaplan, Ofer¹  Navon, Gil¹ 

[1] Medicine Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA

关键词: NMR;    13C-;    Deoxy-D-glucose;    2-;    Phosphorylation;    Transport;    Labeling;    13C;    Glycolysis;    (Cancer cell;    Tumor);   

DOI  :  10.1016/0014-5793(89)81246-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We describe the use of 2-deoxy-D-[6-¹³C]glucose to follow simultaneously, by ¹³C NMR, both transport and phosporylation to its 6-phosphate form, in MCF-7 breast cancer cells in vitro and in vivo in subcutaneous tumors in nude mice.

【 授权许可】

Unknown