FEBS Letters | |
Enzymatic synthesis of dihydrosirohydrochlorin (precorrin‐2) and of a novel pyrrocorphin by uroporphyrinogen III methylase | |
Warren, Martin J.2  Scott, A.Ian2  Sowa, Blair A.1  Roessner, Charles A.2  Santander, Patricio J.2  Stolowich, Neal J.2  | |
[1] Centre for Biological N M R, Department of Veterinary Pathology, Texas A & M University, College Station, TX 77843, USA;Centre for Biological N M R, Department of Chemistry, Texas A & M University, College Station, TX 77843, USA | |
关键词: Uroporphyrinogen III methylase; Dihydrosirohydrochlorin; Precorrin-2; Dipyrrocorphin; Pyrrocorphin; NMR; 13C-; uro'gen; uroporphyrinogen; SAM; S-adenosyl methionine; SUMT; S-adenosyl methionine uroporphyrinogen methyl transferase; NMR; nuclear magnetic resonance; HPLC; high pressure liquid chromatography; pbg; porphobilinogen; | |
DOI : 10.1016/0014-5793(90)80640-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Uroporphyrinogen III methylase was purified from a recombinant hemB− strain of E. coli harbouring a plasmid containing the cysG gene. N-terminal analysis of this purified protein gave an amino acid sequence corresponding to that predicted from the genetic code. From the u.v./visible spectrum of the reaction catalysed by this SAM dependent methylase it was possible to observe the sequential appearance of the chromophores of a dipyrrocorphin and subsequently of a pyrrocorphin. Confirmation of this transformation was obtained from 13C-NMR studies when it was demonstrated, for the first time directly, that uroporphyrinogen is initially converted into dihydrosirohydrochlorin (precorrin-2) and then, by further methylation, into a novel trimethylpyrrocorphin.
【 授权许可】
Unknown
【 预 览 】
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