Kidney fatty acid‐binding protein: Identification as α2U‐globulin

FEBS Letters

Kimura, Hideka²  Odani, Shoji¹  Suzuki, Jun-ichi¹  Ono, Teruo¹  Arakawa, Masaai¹ 

[1] Department of Biochemistry, Niigata University School of Medicine, Niigata 951, Japan

关键词: Fatty acid-binding protein;    Globulin;    α2U-;    Male-specific protein;    (Rat;    Kidney);    FABP;    fatty acid-binding protein;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    HPLC;    high-performance liquid chromatography;    TFA;    trifluoroacetic acid;   

DOI  :  10.1016/0014-5793(89)80261-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A fatty acid-binding protein was purified from male rat kidney by the method of Fujii et al. [(1987) Arch. Biochem. Biophys. 254, 552–558]. Based on the close similarity of amino acid composition and the identity of the amino acid sequence in the amino-terminal region, the fatty acid-binding protein was identified as α_2U-globulin, which is the major male-specific protein in rat urine. The amino-terminal residue of the isolated kidney protein corresponds to residue 10 of α_2U-globulin, suggesting that the protein has been proteolytically processed in the kidney. This fatty acid-binding protein could not be immunologically detected in female rat kidney.

【 授权许可】

Unknown