Reduced lateral mobility of a fluorescent lipid analog in cell membranes of rat fibroblasts transformed by simian virus 40

FEBS Letters

Miyakawa, Tokichi¹  Kawasaki, Yukishige¹  Sakaguchi, Kenji¹  Zundel, Jean-Luc¹ 

[1]Mitsubishi-Kasei Institute of Life Sciences, 11, Minamiooya, Machida-shi, Tokyo 194, Japan

关键词: Virus-induced transformation;    Fluorescence photobleaching recovery;    Lateral motion;    (Fibroblast);    S-F-concanavalin A;    succinyl concanavalin A conjugated with fluorescein isothiocyanate;    F-concanavalin A;    concanavalin A conjugated with fluorescein isothiocyanate;    F18;    5-(octadecylthiocarbamoylamino)fluorescein;    SV40;    simian virus 40;    FPR;    fluorescence photobleaching recovery;   

DOI  :  10.1016/0014-5793(89)80254-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In order to study the difference between normal and transformed cells, lateral motion of fluorescent molecules embedded into cell membranes of rat clonal fibroblasts and its SV40-transformed derivative cells was measured by the FPR technique. The lateral diffusion coefficient of a fluorescent fatty acid analog, F18, was smaller in transformed cells than normal cells. This indicates that the lipid phase of membranes from transformed cells is less fluid than that from normal cells. On the other hand, the lateral diffusion coefficient of S-F-concanavalin A was identical in both cells. These results suggest that the mobility of different molecules on the membranes is controlled by different mechanisms.

【 授权许可】

Unknown