Lateral motion of fluorescent molecules embedded into cell membranes of clonal myogenic cells, L6, changes upon cell maturation

FEBS Letters

Kawasaki, Yukishige¹  Seto-Ohshima, Akiko¹  Wakayama, Nobuyuki¹ 

[1] Mitsubishi-Kasei Institute of Life Sciences, 11, Minamiooya, Machida, Tokyo 194, Japan

关键词: Lateral motion;    Fluorescence photobleaching recovery;    Cell fusion;    Maturation;    (Myogenic cell);    S-F-ConA;    succinyl concanavalin A conjugated with fluorescein isothiocyanate;    F-ConA;    concanavalin A conjugated with fluorescein isothiocyanate;    F18;    5-(octadecylthiocalbamoylamino)fluorescein;    FPR;    fluorescence photobleaching recovery;    PBS;    phosphate buffered saline;   

DOI  :  10.1016/0014-5793(88)80842-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The lateral motion of fluorescent molecules embedded into cell membranes of myogenic cell line, L6, was measured. The motion of S-F-ConA became faster at cell fusion stage, and became slower after fusion. On the other hand, the motion of lipid analog, F18, was not changed at cell fusion stage. However, after fusion when myotubes were formed, the motion of F18 became slower. At cell fusion stage, there was a large variation in the motion of S-F-ConA. This means that at this stage the properties of myoblasts change drastically and rapidly.

【 授权许可】

Unknown