期刊论文详细信息
FEBS Letters
The effect of cell synchronization on the efficiency of stable gene transfer by electroporation
Tsuruta, Satoru1  Mikawa, Haruki1  Yorifuji, Tohru1 
[1] Department of Pediatrics, Faculty of Medicine, Kyoto University, 54 Shogoin Sakyo, Kyoto 606, Japan
关键词: Cell synchronization;    Transformation;    Hydroxyurea;    Aphidicolin;    Electroporation;    (Ltk− cell);   
DOI  :  10.1016/0014-5793(89)80221-2
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

We synchronized thymidine kinase deficient mouse Ltk cells by two different methods, hydroxyurea double-block treatment or aphidicolin single-block treatment and transformed them with the cloned herpes simplex virus thymidine kinase gene at various time intervals by the electroporation technique. Marked enhancement of stable transformation efficiency was observed at the time corresponding to the peak of G2/M phase. These results suggest that the G2/M phase is the most efficient period for stable gene transfer by electroporation.

【 授权许可】

Unknown   

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