The effect of cell synchronization on the efficiency of stable gene transfer by electroporation

FEBS Letters

Tsuruta, Satoru¹  Mikawa, Haruki¹  Yorifuji, Tohru¹ 

[1] Department of Pediatrics, Faculty of Medicine, Kyoto University, 54 Shogoin Sakyo, Kyoto 606, Japan

关键词: Cell synchronization;    Transformation;    Hydroxyurea;    Aphidicolin;    Electroporation;    (Ltk− cell);   

DOI  :  10.1016/0014-5793(89)80221-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We synchronized thymidine kinase deficient mouse Ltk⁻ cells by two different methods, hydroxyurea double-block treatment or aphidicolin single-block treatment and transformed them with the cloned herpes simplex virus thymidine kinase gene at various time intervals by the electroporation technique. Marked enhancement of stable transformation efficiency was observed at the time corresponding to the peak of G₂/M phase. These results suggest that the G₂/M phase is the most efficient period for stable gene transfer by electroporation.

【 授权许可】

Unknown