The human 32‐kDa stress protein induced by exposure to arsenite and cadmium ions is heme oxygenase

FEBS Letters

Tokunaga, Rikio¹  Yoshinaga, Takeo²  Taketani, Shigeru¹  Kohno, Hirao¹ 

[1] Department of Hygiene, Kansai Medical University, Moriguchi, Osaka 570 Japan;Department of Public Health, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606, Japan

关键词: Stress protein;    32 kDa;    Heme oxygenase;    Arsenite;    Cadmium ion;    (Human cell);    MEM;    Eagle's minimum essential medium;   

DOI  :  10.1016/0014-5793(89)80215-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Exposure of HeLa and HL60 cells to sodium arsenite or cadmium chloride led to marked increases in cellular heme oxygenase activity. SDS-polyacrylamide gel clectrophoresis of [^35S]methionine-labeled cellular proteins indicated that these treatments also resulted in the induction of a 32-kDa protein. Immunoblot analysis further showed that the 32-kDa protein reacted with anti-bovine heme oxygenase antibodies. Treatment of the cells with cobaltic chloride or heat induced neither the 32-kDa protein nor heme oxygenase activity. It is concluded that the 32-kDa stress protein induced by arsenite and cadmium ions in these human cells is heme oxygenase.

【 授权许可】

Unknown