FEBS Letters | |
The human 32‐kDa stress protein induced by exposure to arsenite and cadmium ions is heme oxygenase | |
Tokunaga, Rikio1  Yoshinaga, Takeo2  Taketani, Shigeru1  Kohno, Hirao1  | |
[1] Department of Hygiene, Kansai Medical University, Moriguchi, Osaka 570 Japan;Department of Public Health, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606, Japan | |
关键词: Stress protein; 32 kDa; Heme oxygenase; Arsenite; Cadmium ion; (Human cell); MEM; Eagle's minimum essential medium; | |
DOI : 10.1016/0014-5793(89)80215-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Exposure of HeLa and HL60 cells to sodium arsenite or cadmium chloride led to marked increases in cellular heme oxygenase activity. SDS-polyacrylamide gel clectrophoresis of [35S]methionine-labeled cellular proteins indicated that these treatments also resulted in the induction of a 32-kDa protein. Immunoblot analysis further showed that the 32-kDa protein reacted with anti-bovine heme oxygenase antibodies. Treatment of the cells with cobaltic chloride or heat induced neither the 32-kDa protein nor heme oxygenase activity. It is concluded that the 32-kDa stress protein induced by arsenite and cadmium ions in these human cells is heme oxygenase.
【 授权许可】
Unknown
【 预 览 】
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