Identification of both Gi2 and a novel, immunologically distinct, form of Go in rat myometrial membranes

FEBS Letters

Unson, Cecilia²  Tanfin, Zahra¹  Milligan, Graeme³  Harbon, Simone¹  Goureau, Olivier¹ 

[1] Endocrinologie et Regulations Cellulaires, Université Paris Sud, 91405 Orsay Cedex, France;Department of Biochemistry, The Rockefeller University, New York, NY 10021, USA;Molecular Pharmacology Group, Departments of Biochemistry and Pharmacology, University of Glasgow, Glasgow G12 8QQ, Scotland

关键词: Guanine nucleotide-binding protein;    Pertussis toxin;    (Myometrium;    Brain);    G-protein;    guanine nucleotide-binding protein;    Go;    a G-protein of unknown function which is particularly abundant in brain;    Gi1;    Gi2;    Gi3;    highly homologous G-proteins which may be involved in the control of inhibition of adenylate cyclase (these are named for the chronology of identification of cDNA sequences containing the coding potential for these proteins);   

DOI  :  10.1016/0014-5793(89)80574-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Immunoblotting of rat myometrial membranes with an antiserum (SG1) which recognizes the α-subunits of both G_i1 and G_i2 indicated the presence of detectable levels of an apparently single form of some 40 kDa. A second antiserum (LE2) specific for G_i2 also recognized this protein, confirming its identity. Immunoblotting of the myometrial membranes with a series of antipeptide (OC1, IM1, ON1) and polyclonal (RV3) antisera, all of which recognize rat brain G_o, produced a more complex pattern. Antisera OC1 and ON1 recognized a single polypeptide which essentially comigrated with rat brain G_o. In contrast, antisera RV3 and IM1 did not recognize the myometrial protein. These data provide evidence for the presence of G_i2 and of a novel G-protein, related immunologically to G_o, in rat myometrial membranes.

【 授权许可】

Unknown