Ischemia‐induced changes in myocardial paramagnetic metabolites: Implications for intracellular oxy‐radical generation

FEBS Letters

Kalyanaraman, B.¹  Baker, John E.² 

[1] National Biomedical Electron Spin Resonance Center, Department of Radiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA;Department of Cardiothoracic Surgery, Medical College of Wisconsin, Milwaukee, WI 53226 USA

关键词: Ischemia;    Iron-sulfur center;    Ubisemiquinone;    ESR;    Oxy-radical;    (Rat heart);   

DOI  :  10.1016/0014-5793(89)80552-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Evidence to identify the cellular sources of oxy-radical generation in myocardium has been of an indirect nature. We have used low-temperature ESR spectroscopy to identify and characterize ischemia-induced changes in myocardial paramagnetic metabolites. Iron-sulfur proteins associated with the NADH or succinate dehydrogenases of the mitochondrial electron-transport chain were progressively reduced with the onset and development of ischemia. This study provides direct evidence for ischemia-induced changes in an intracellular source of superoxide radical generation that may contribute to oxy-radical production during reperfusion.

【 授权许可】

Unknown