Enhanced levels of cathepsin B mRNA in murine tumors

FEBS Letters

Moin, Kamiar³  Sloane, Bonnie F.³  Honn, Kenneth V.²  Sanders, Virginia J.³  Fong, Dunne¹  McKernan, Timothy B.³  Rozhin, Jurij³ 

[1] Department of Biological Sciences, Rutgers University, Piscataway, NJ 08854, USA;Department of Radiation Oncology, Wayne State University, Detroit, MI 48201, USA;Department of Pharmacology, Wayne State University, Detroit, MI 48201, USA

关键词: Cysteine proteinase;    Acid hydrolase;    Tumor metastasis;    Gene expression;   

DOI  :  10.1016/0014-5793(89)81162-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Relative amounts of mRNA for cathepsin B were measured in normal murine liver and three murine tumors, an invasive liver tumor (hepatoma, Hepa cl 9) and two melanoma variants (B16-F1 and B16 amelanotic melanoma, B16a). Using a human cDNA to the cathepsin B coding region as a hybridization probe, we detected two species of cathepsin B specific RNA transcripts (2.2 and 4.1 kb) in total RNA preparation of all four tissues. The concentrations of the 2.2 and 4.1 kb species were 3.6 and 2.7-fold greater in the highly metastatic B16a melanoma than in normal liver. The concentration of the 2.2 kb species in the invasive hepatoma wa 1.7-fold greater than in normal liver. The increased levels of the 2.2 kb message were reflected in increases in activity of cathepsin B in both Hepa cl 9 and B16a.

【 授权许可】

Unknown